COX Fluorescent Activity Assay Kit-荧光法检测试剂盒-ELISA_抗体_蛋白_核苷酸

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COX Fluorescent Activity Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：700200

规格：2 x 96 wells

目录价：￥6104

产品详情

产品名称:

COX Fluorescent Activity Assay Kit

别名:

Cyclooxygenase Fluorescent Activity Assay Kit

产品描述:

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both COX and peroxidase activities. The COX component converts arachidonic acid to a hydroperoxy endoperoxide (PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (PGH2), the precursor of PGs, thromboxanes, and prostacyclins.{500,501} It is now well established that there are two distinct isoforms of COX, namely COX-1 and COX-2. Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin that can be analyzed using an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.

保存温度:

-80

运输温度:

-80

有效期:

365

来源:

Animal/Sheep

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