SouthernBiotech(Southern Biotechnology Associates, Inc)是由美国阿拉巴马大学伯明翰分校(UAB)Dr. Max Cooper细胞免疫学实验室中的科学家们于1982年创立,成立30年来一直致力于生产高质量、高亲和纯化的二抗、标记二抗以及其它免疫试剂,包括多种类型的多克隆和单克隆抗体、胶原蛋白和其它细胞外基质蛋白。
欣博盛生物自成立以来,一直是SouthernBiotech授权优质代理商。如您需购买SouthernBiotech公司的产品或咨询其它问题,欢迎联系我们。
多克隆二抗&试剂 | 细胞凋亡 |
Anti: Sheep, Human, Turkey, Bovine, Rhesus, Chicken, Porcine, Hamster, Canine, Mouse, Rat, Equine, Goat, Rabbit, Feline, Guinea Pig, Streptavidin and Neutralite Avidin | ELISA Sets, ApoScreen™, Recombinant Proteins and Antibodies |
单克隆二抗 | 促炎症反应 |
Anti: Human, Rhesus, Chicken, Hamster, Mouse, Rat, Goat and Rabbit | ELISA Sets, Recombinant Proteins and Antibodies |
物种特异性二抗 | 免疫检测试剂盒 |
Rhesus, Mouse, Goat and Rabbit | Clonotyping/Isotyping, Human Cytokines, Mouse Cytokines and Apoptosis Detection |
血清白蛋白抗体 | 纯化的胞外基质蛋白及抗体 |
Bovine | Collagen, Laminin, Antibodies, Fibronectin |
CD分子和其他细胞表面蛋白抗体 | 细胞信号传导 |
Anti: Human, Chicken, Porcine, Mouse, Rabbit, Feline | Human, Chicken, Porcine, Mouse, Rat and Feline |
同型对照 | 酶底物 |
Species: Sheep, Human, Rhesus, Chicken, Donkey, Porcine, Hamster, Canine, Mouse, Rat, Goat and Rabbit | 病毒蛋白 |
表观遗传&核信号传导 | 缓冲液 |
Human and Mouse | 其他 |
细胞因子 | Mycoplasma Detection Kit, Keratin and Anti-Biotin |
SBA单抗分型多功能检测试剂盒 -----分型首选!
产品编号:5300-04 (B)/ 5300-05 (B)/ 5300-01 (B)
在单克隆抗体的制备过程中,杂交瘤细胞的筛选和鉴别是最重要的步骤。首先需要筛选出正确融合的杂交瘤细胞,再筛选出能分泌目标抗体的杂交瘤细胞,最终将筛选出来的杂交瘤细胞进行扩大培养,制备大量单克隆抗体。在进行能分泌目标抗体杂交瘤细胞的筛选时,需要鉴定这类杂交瘤细胞培养上清液中单克隆抗体的类或亚类,最常用的是ELISA法,因其快速、简便、灵敏度高、特异性强、易于标准化等优点,已得到广泛应用。
实验原理
单克隆抗体分型ELISA试剂盒运用双抗体夹心法原理,先用Ig捕获二抗在96孔板上包被固相,然后加入待测的杂交瘤细胞培养上清液,培养上清液中的目标蛋白便会与96孔板上固相二抗结合,洗板去除游离物质,然后加入用于筛选杂交瘤细胞的标记二抗孵育,洗板去除游离物质,加入显色底物显色,在450nm处测定OD值,进行定性及定量分析。
试剂盒特点
● 操作流程简单,无需复杂的处理步骤,结果准确且易于分析;
● 多功能性,可用于初筛抗体高分泌杂交瘤细胞株,鉴定阳性克隆细胞的特异性抗体分型(IgA、IgM、IgG1、IgG2a、IgG2b、IgG3), 鉴定抗体kappa、lambda轻链异构体以及普通抗体分型ELISA研究;
● 性价比高,搭配使用可进行1000次以上样本检测;
● 严格的QC检测,确保批间一致性。
产品信息 (可在本站搜索产品编号,直接查看产品详情)
产品名称 | 产品编号 | 产品说明 |
Mouse Immunoglobulin Panel | 5300-01 | 100ug*6种免疫球蛋白 |
B6/C57J Mouse Immunoglobulin Panel | 5300-01B | 100ug*6种免疫球蛋白 |
SBA Clonotyping System-AP | 5300-04 | 超过1000次HRP酶分析 |
SBA Clonotyping System-B6/C57J-AP | 5300-04B | 超过1000次HRP酶分析 |
SBA Clonotyping System-HRP | 5300-05 | 超过1000次AP酶分析 |
SBA Clonotyping System-B6/C57J-HRP | 5300-05B | 超过1000次AP酶分析 |
低内毒素无叠化合物二抗
内毒素是革兰氏阴性细菌细胞壁中的一种成分,也叫脂多糖,对宿主有毒性,当细菌死亡溶解或用人工方法破坏菌细胞后便释放出来,其毒性成分主要为类脂质A,耐热稳定,低内毒素是指产品含有非常微量的内毒素,是细胞和组织培养的理想选择。叠氮钠(Sodium azide),白色粉末,分子式NaN3 ,熔点275°C,沸点300°C,剧毒,和氰化物相似,对细胞色素氧化酶和其它酶有抑制作用,并能使体内氧合血红蛋白形成受阻,有显著的降压作用,对眼和皮肤有刺激性,如吸入、口服或经皮肤吸收,可引起中毒死亡,可用于血清防腐。无叠氮钠指产品中不含叠氮钠成分。
产品名称 | 编号 | 抗体类型 | 宿主来源 | 规格 |
Mouse IgM-LE/AF | 0101-14 | Monoclonal | Mouse | 0.5 mg |
Mouse IgM-LE/AF | 0102-14 | Monoclonal | Mouse | 0.5 mg |
Mouse IgG2a-LE/AF | 0103-14 | Monoclonal | Mouse | 0.5 mg |
Mouse IgG2b-LE/AF | 0104-14 | Monoclonal | Mouse | 0.5 mg |
Mouse IgG3-LE/AF | 0105-14 | Monoclonal | Mouse | 0.5 mg |
Mouse IgA-LE/AF | 0106-14 | Monoclonal | Mouse | 0.5 mg |
Mouse IgG-LE/AF | 0107-14 | Polyclonal | Mouse | 0.5 mg |
Rat IgG-LE/AF | 0108-14 | Polyclonal | Rat | 0.5 mg |
Mouse F(ab’)2 IgG1-LE/AF | 0115-14 | Monoclonal | Mouse | 0.5 mg |
Rat IgG1-LE/AF | 0116-14 | Monoclonal | Rat | 0.5 mg |
Rat IgG2a-LE/AF | 0117-14 | Monoclonal | Rat | 0.5 mg |
Rat IgG2b-LE/AF | 0118-14 | Monoclonal | Rat | 0.5 mg |
Rat IgM-LE/AF | 0120-14 | Monoclonal | Rat | 0.5 mg |
Hamster IgG-LE/AF | 0121-14 | Polyclonal | Hamster | 0.5 mg |
Rat Anti-Mouse CD2/LFA-2-LE/AF | 1525-14 | Monoclonal | Rat | 0.5 mg |
Hamster Anti-Mouse CD3e-LE/AF | 1530-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Hamster Anti-Mouse CD3e-LE/AF | 1531-14 | Monoclonal | Hamster (Syrian) | 0.5 mg |
Rat Anti-Mouse CD3e-LE/AF | 1535-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD8a/Ly-2-LE/AF | 1550-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD11b/Mac-1a-LE/AF | 1561-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD19-LE/AF | 1575-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD25/IL-2Ra-LE/AF | 1600-14 | Monoclonal | Rat | 0.5 mg |
Hamster Anti-Mouse CD28-LE/AF | 1610-14 | Monoclonal | Hamster (Syrian) | 0.5 mg |
Hamster Anti-Mouse CD28-LE/AF | 1615-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Hamster Anti-Mouse CD30-LE/AF | 1620-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Rat Anti-Mouse CD38-LE/AF | 1635-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD38-LE/AF | 1640-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD40-LE/AF | 1645-14 | Monoclonal | Rat | 0.5 mg |
Hamster Anti-Mouse CD154/CD40L-LE/AF | 1650-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Rat Anti-Mouse CD54/ICAM-1-LE/AF | 1701-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD62L/L-selectin-LE/AF | 1705-14 | Monoclonal | Rat | 0.5 mg |
Hamster Anti-Mouse CD69/VEA-LE/AF | 1715-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Rat Anti-Mouse CD80/B7-1-LE/AF | 1730-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD86/B7-2-LE/AF | 1735-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD90/Thy-1-LE/AF | 1740-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD90.2/Thy-1.2-LE/AF | 1750-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse Ly-6A/E-LE/AF | 1755-14 | Monoclonal | Rat | 0.5 mg |
Hamster Anti-Mouse TCRgd-LE/AF | 1780-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Hamster Anti-Mouse TCRb-LE/AF | 1785-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Hamster Anti-Mouse CD152/CTLA-4-LE/AF | 1790-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Hamster Anti-Mouse CD152/CTLA-4-LE/AF | 1791-14 | Monoclonal | Hamster (Syrian) | 0.5 mg |
Mouse Anti-Mouse NK1.1-LE/AF | 1805-14 | Monoclonal | Mouse | 0.5 mg |
Hamster Anti-Mouse CD81-LE/AF | 1825-14 | Monoclonal | Hamster (Armenian) | 0.5 mg |
Rat Anti-Mouse MAdCAM-1-LE/AF | 1865-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse LPAM-1-LE/AF | 1870-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD117/c-kit-LE/AF | 1880-14 | Monoclonal | Rat | 0.5 mg |
Mouse Anti-Mouse I-Ek-LE/AF | 1897-14 | Monoclonal | Mouse | 0.5 mg |
Rat Anti-Mouse Ly-6G/GR-1-LE/AF | 1900-14 | Monoclonal | Rat | 0.5 mg |
Mouse Anti-Mouse H-2Db-LE/AF | 1910-14 | Monoclonal | Mouse | 0.5 mg |
Mouse Anti-Mouse H-2Db-LE/AF | 1913-14 | Monoclonal | Mouse | 0.5 mg |
Rat Anti-Mouse CD86/B7-2-LE/AF | 1915-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse CD102/ICAM-2-LE/AF | 1925-14 | Monoclonal | Rat | 0.5 mg |
Mouse F(ab’)2 Anti-Rat IgM-LE/AF | 3082-14 | Monoclonal | Mouse | 0.5 mg |
Mouse Anti-Human CD9-LE/AF | 9310-14 | Monoclonal | Mouse | 0.5 mg |
Mouse Anti-Human CD11a/LFA-1a-LE/AF | 9320-14 | Monoclonal | Mouse | 0.5 mg |
Mouse Anti-Human CD3-LE/AF | 9515-14 | Monoclonal | Mouse | 0.5 mg |
Mouse Anti-Human CD95/Fas-LE/AF | 9730-14 | Monoclonal | Mouse | 0.5 mg |
Mouse Anti-Human CD95/Fas-LE/AF | 9735-14 | Monoclonal | Mouse | 0.5 mg |
Rat Anti-Human IL-10-LE/AF | 10100-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Human GM-CSF-LE/AF | 10111-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Human IL-3-LE/AF | 10115-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Human IL-4-LE/AF | 10117-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Human IL-7-LE/AF | 10122-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Human IL-13-LE/AF | 10125-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Human G-CSF-LE/AF | 10127-14 | Monoclonal | Rat | 0.5 mg |
Rat Anti-Mouse IL-4-LE/AF |
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更多SouthernBiotech产品信息请咨询中国一级代理商欣博盛生物
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