官网链接
折扣/货期| 货号：701350 | 规格：96 wells | 目录价：￥5292 |
产品详情
产品名称:
α-Ketoglutarate Detection Assay Kit
别名:
2-Oxoglutarate|α-KG
产品描述:
Cayman’s α-KG Detection Assay provides fluorometric- or colorimetric-based methods for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular and extracellular α-KG concentrations in cell culture samples. In this assay, alanine transaminase (ALT) deaminates α-KG to form pyruvate.{33073,33074} Pyruvate is then oxidized by pyruvate oxidase to yield acetyl phosphate, hydrogen peroxide (H2O2), and carbon dioxide.{33075} As H2O2 is produced, the cycling mechanism of horseradish peroxidase (HRP) results in the concurrent reduction of H2O2 to H2O and the oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin.{33076} Resorufin fluorescence is analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.
保存温度:
-20
运输温度:
-20
有效期:
730
来源:
Animal/Bovine
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