货号:GTX27901 | 规格:100μg | 目录价:¥询价 |
产品详情
产品名称:
NIH-3T3 whole cell lysate
产品描述:
NIH-3T3 embryonic cells grown to 85% confluency were harvested, homogenized in modified RIPA buffer (150 mM NaCl, 50 mM Tris-Cl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1 mM EDTA, 1 mM PMSF, 5 μg/ml Pepstatin A, 2 μg/ml Leupeptin, 1 mM sodium orthovanadate, 1 mM sodium fluoride.), and cleared by centrifugation. Total protein concentration of the lysate was determined using BCA protein assay (BSA was used as a standard). Lysate was mixed with Laemmli Sample Buffer containing reducing reagent and was boiled for 5 minutes. Lysate is supplied as 200 μg in 100 μl per vial (2.0 mg/ml total protein concentration) in 1X Laemmli sample buffer.
克隆号:
纯化方式:
Whole Cell Lysate
产品浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
保存温度:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
运输温度:
dry ice
产品形式:
Liquid
存储溶液:
1x SDS sample buffer, 5% β-mercaptoethanol, no preservative.
产地:
美国
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