NIH-3T3 whole cell lysate-细胞和组织裂解液-ELISA_抗体_蛋白_核苷酸

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NIH-3T3 whole cell lysate

品牌：GeneTex

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说明书

折扣/货期

货号：GTX27901

规格：100μg

目录价：￥询价

产品详情

产品名称:

NIH-3T3 whole cell lysate

产品描述:

NIH-3T3 embryonic cells grown to 85% confluency were harvested, homogenized in modified RIPA buffer (150 mM NaCl, 50 mM Tris-Cl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1 mM EDTA, 1 mM PMSF, 5 μg/ml Pepstatin A, 2 μg/ml Leupeptin, 1 mM sodium orthovanadate, 1 mM sodium fluoride.), and cleared by centrifugation. Total protein concentration of the lysate was determined using BCA protein assay (BSA was used as a standard). Lysate was mixed with Laemmli Sample Buffer containing reducing reagent and was boiled for 5 minutes. Lysate is supplied as 200 μg in 100 μl per vial (2.0 mg/ml total protein concentration) in 1X Laemmli sample buffer.

克隆号:

纯化方式:

Whole Cell Lysate

产品浓度:

1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:

dry ice

产品形式:

Liquid

存储溶液: