产品描述:
Zika virus (ZIKV) is a mosquito-borne, positive-stranded RNA virus and a member of the Flavivirus genus.{31923,72406} ZIKV infection is associated with fever, rashes, and conjunctivitis, as well as more severe symptoms, which include Guillain-Barré syndrome in adults and microcephaly or congenital malformations in fetuses and newborns.{31923,72408} The single-stranded RNA genome of ZIKV is translated as a polypeptide, which is cleaved by host and viral proteases into structural capsid (C), precursor membrane (prM), and envelope (E) proteins and seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5.{31923,72407} ZIKV E protein is composed of a structural domain, a dimerization domain, which contains a membrane fusion loop, and a structural domain containing a hinge region, two transmembrane helices, and a host receptor-binding region.{72406} It is a homodimer in the viral membrane but transitions to a homotrimer state in host cell organelles with low pH, in which viral replication and assembly occurs, as well as where E protein can form a heterodimer with prM.{72407} ZIKV E protein glycosylation at an N-terminal asparagine is necessary for ZIKV infectivity and optimal replication in cells and mice.{72408} Cayman's Zika Virus Envelope Protein (strain Zika SPH2015) (T366A, Q367G, W391R, L397R mutant; recombinant) protein consists of 405 amino acids, has a calculated molecular weight of 45.6 kDa, and a predicted N-terminus of Ile291 after signal peptide cleavage.