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CUTANA™ pAG-Tn5 for CUT&Tag
品牌： EpiCypher
产品货号： 15-1017
规格： 50 Reactions

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  • 产品名称:
    CUTANA™ pAG-Tn5 for CUT&Tag
  • 别名:
  • 产品描述:
    CUTANA pAG-Tn5 is the key reagent for performing efficient mapping of chromatin features in Cleavage Under Targets and Tagmentation (CUT&Tag) [1]. CUT&Tag offers significant improvements in signal to noise at reduced cell inputs and sequencing depth compared to ChIP-seq. As a fusion of Proteins A and G to a highly active E. coli transposase mutant (Tn5), CUTANA pAG-Tn5 is compatible with target antibodies from a broad spectrum of host species. It also comes without an epitope tag, making it suitable for tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, V5, etc.). This product is highly purified to remove contaminating E. coli DNA, which can be tagmented during storage and complicate analysis at low cell numbers. For superior normalization as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. The Tn5 comes charged with mosaic adapters and is ready to be used in CUT&Tag.
  • 反应种属:
  • 宿主来源:
    E. coli
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  • 表达系统:
    E. coli
  • 阳性对照:
  • 保存温度:
    Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
  • 运输温度:
    DO NOT FREEZE!! Frozen cold packs.
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  • 产品形式:
    50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol
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  • 产地:
    美国
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