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Alpha Synuclein A90C Mutant Monomers
品牌： Stressmarq
产品货号： SPR-478B
规格： 100 µg

报价：
折后价：

数量： - +
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  • 产品名称:
    Alpha Synuclein A90C Mutant Monomers
  • 别名:
    SNCA, alpha-synuclein, synuclein, Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, SNCA protein
  • 产品描述:
    Human Recombinant Alpha Synuclein A90C Mutant Monomers
  • 反应种属:
    Human
  • 宿主来源:
  • 实验应用:
    WB | SDS PAGE | In vitro Assay | Conjugation | FRET
  • 靶标/特异性:
  • 同种型:
  • 推荐稀释度:
  • 免疫原:
  • 免疫原种属:
  • 克隆性:
  • 克隆号:
  • 纯化方式:
    Ion-exchange Purified
  • 偶联:
    No Tag
  • 产品浓度:
    2 mg/ml
  • 表达系统:
    E. coli
  • 阳性对照:
  • 保存温度:
    -80ºC
  • 运输温度:
    Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
  • 细胞定位:
  • 预期分子量:
    14.49 kDa
  • 产品形式:
  • 存储溶液:
    20mM Hepes pH 7.4, 150mM NaCl, 1mM TCEP pH 7.0
  • 有效期:
  • 产地:
    加拿大
  • 外观:
  • 生产商:
  • 功能与背景:
    Thioflavin-T (ThT) fluorescence remains a common measurement of alpha-synuclein fibril formation, yet ThT exhibits poor affinity for oligomers and early aggregates. The alpha-synuclein A90C mutant monomers can be specifically labelled with alternative fluorophores (such as Alexa 488/Alexa 647) via maleimide chemistry to enable more sensitive FRET analysis of aggregation. The A90C mutant showed no perturbation of monomer structure and Alexa Fluor dye attachment to cysteine 90 was demonstrated to have no effect on the kinetics of fibril formation (1-3). Residue 90 is at the periphery of the NAC region, a key constituent of the alpha-synuclein β-sheet fibril core, which results in fluorophores on different monomers coming into close proximity upon formation of β-sheet structure during aggregation (4). Note - to prevent the potential mis-translation of alpha-synuclein Y136 as C136 during E.coli expression, the Y136-TAT construct was used (5).
  • URL:
  • 灵敏度:
  • 检测范围:
  • 检测方法:
  • 样本类型:
    20mM Hepes pH 7.4, 150mM NaCl, 1mM TCEP pH 7.0
  • 产品特点:
  • 纯度:
    >95%
  • 生物活性:
  • 内毒素水平:
  • 来源:
  • 表达方式:
  • Accession #:
  • CAS号:
  • 分子式:
  • 溶解度:
  • PubChem CID:
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