Alpha Synuclein E114C Mutant Monomers: ATTO 488 - 深圳欣博盛生物科技有限公司

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Alpha Synuclein E114C Mutant Monomers: ATTO 488

品牌： Stressmarq

产品货号： SPR-517E-A488

规格： 100 µg x5

报价：

折后价：

数量： - +

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产品名称:

Alpha Synuclein E114C Mutant Monomers: ATTO 488
别名:

Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein
产品描述:

Human Recombinant Alpha Synuclein E114C Mutant Monomers: ATTO 488
反应种属:

Human
宿主来源:
实验应用:

WB | Native PAGE | In vitro Assay | In vivo Assay
靶标/特异性:
同种型:
推荐稀释度:
免疫原:
免疫原种属:
克隆性:
克隆号:
纯化方式:

Ion-exchange & SEC purified
偶联:

ATTO 488
产品浓度:

Lot/batch specific. See included datasheet.
表达系统:

E.coli
阳性对照:
保存温度:

-80ºC
运输温度:

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
细胞定位:
预期分子量:

14.434 kDa
产品形式:
存储溶液:

1X PBS pH 7.4
有效期:
产地:

加拿大
外观:
生产商:
功能与背景:

The alpha-synuclein (aSyn) E114C mutation facilitates a single site-specific conjugation with ATTO-488 maleimide that avoids any hindrance on fibrilization or cell entry that may be conferred by non-specific lysine targeting conjugations. This conjugation is ideal due to internal position relative to C-terminal truncation sites, proximity to the NAC, and lack of interference with recruitment in vitro or in primary neurons (1, 2). Pre-formed fibrils (PFFs) generated with 5-25% fluorescently tagged E114C mutants have demonstrated a relative potency >80% compared to wild-type aSyn for inducing misfolding of endogenous aSyn, indicating no significant perturbation of seeding in living cells (1). Atto-488 is a useful tool for identifying cell entry, as the addition of Trypan Blue to cultures prior to imaging will quench fluorescence of extracellular Atto-488 conjugated aSyn (3). Our aSyn E114C-Atto-488 PFFs, which contain 10% fluorescently tagged E114C mutants, are an excellent tool for studying cell entry and localization, with demonstrated entry into neurons after trypan blue quenching.
URL:
灵敏度:
检测范围:
检测方法:
样本类型:

1X PBS pH 7.4
产品特点:
纯度:

>95%
生物活性:
内毒素水平:
来源:
表达方式:
Accession #:
CAS号:
分子式:
溶解度:
PubChem CID: