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CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag
品牌： EpiCypher
产品货号： 15-1028
规格： 75 µL

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折后价：

数量： - +
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  • 产品名称:
    CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag
  • 别名:
  • 产品描述:
    Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support. CUTANA™ Uncharged 6xHis-pAG-Tn5 is a fusion of proteins A and G to E.coli transposase (Tn5), the key enzyme for CUT&Tag [1]. The fusion protein contains a polyhistidine tag (6xHis) to enable applications that require affinity purification steps (for an untagged version of uncharged pAG-Tn5, see EpiCypher 15-1025). Uncharged Tn5 must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag (see reference [1] and Application Notes). His-pAG-Tn5 enables purification of differentially barcoded antibody-Tn5 complexes such as those described in MulTI-Tag (Multiple Target Identification by Tagmentation) and multi-CUT&Tag workflows to simultaneously interrogate multiple chromatin proteins in a single CUT&Tag reaction [2,3]. These approaches have been applied to map histone post-translational modifications and RNA Polymerase II down to single cell resolution to define cell-specific epigenetic landscapes.
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  • 宿主来源:
    E. coli
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  • 表达系统:
    E. coli
  • 阳性对照:
  • 保存温度:
    Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
  • 运输温度:
    DO NOT FREEZE!! Frozen cold packs.
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  • 产品形式:
    50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.
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  • 产地:
    美国
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