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Acidic Patch Mutant H2AE61A Recombinant Nucleosome with Linker DNA, Biotinylated
品牌： EpiCypher
产品货号： 16-2029
规格： 50 µg

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数量： - +
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  • 产品名称:
    Acidic Patch Mutant H2AE61A Recombinant Nucleosome with Linker DNA, Biotinylated
  • 别名:
  • 产品描述:
    The acidic patch is a negatively charged region of the nucleosome surface that serves as a conserved interaction hub for neighboring nucleosomes and chromatin binding proteins, often via salt bridges with arginine anchors [1]. The acidic patch plays a critical role in chromatin condensation and chromatin remodeling [1-3]. Recombinant mononucleosomes containing acidic patch mutations can be used to study the biological functions of the acidic patch. H2AE61A Recombinant Nucleosome with Linker DNA consists of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’ biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence [4] flanked by 26 bp linker sequences as underlined below. Histone H2A contains a glutamate-to-alanine (E-to-A) substitution within the acidic patch at position 61 (H2AE61A). H2AE61 resides in the alpha2 helix, forming a key salt bridge with H4K16, and mediates chromatin binding with factors such as LANA, RCC1, IL-33, Sir3, and HMGN2 [1]. H2AE61A disrupts chromatin remodeling by SMARCA5/SNF2h, an ATPase subunit of the ISWI family of chromatin remodeling complexes [2,3].
  • 反应种属:
  • 宿主来源:
    Human
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  • 表达系统:
    E. coli & synthetic DNA
  • 阳性对照:
  • 保存温度:
    Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
  • 运输温度:
    Dry ice.
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  • 产品形式:
    1.3 mg/mL mononucleosome in 38.4 μL 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. (27.3 μg protein, 50 μg DNA + protein).
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  • 产地:
    美国
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