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产品名称:
HSP60 Protein
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别名:
60kDa chaperonin Protein, cb863 Protein, CPN60 Protein, GROEL Protein, GroEL Homolog Protein, HLD4 Protein, HSP65 Protein, HSPD1 Protein, HuCHA60 Protein, SPG13 Protein
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产品描述:
Active Human Recombinant HSP60 Protein
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反应种属:
Human
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宿主来源:
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实验应用:
WB | SDS-PAGE | ATPase Activity Assay | Functional Assay | ELISA
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靶标/特异性:
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同种型:
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推荐稀释度:
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免疫原:
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免疫原种属:
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克隆性:
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克隆号:
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纯化方式:
Affinity Purified
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偶联:
His tag
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产品浓度:
Lot/batch specific. See included datasheet.
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表达系统:
E. coli
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阳性对照:
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保存温度:
-20ºC
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运输温度:
Blue Ice or 4ºC
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细胞定位:
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预期分子量:
~60 kDa
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产品形式:
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存储溶液:
20mM Phosphate Buffer, 150mM NaCl, 10% glycerol
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有效期:
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产地:
加拿大
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外观:
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生产商:
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功能与背景:
In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
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URL:
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灵敏度:
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检测范围:
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检测方法:
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样本类型:
20mM Phosphate Buffer, 150mM NaCl, 10% glycerol
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产品特点:
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纯度:
>90%
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生物活性:
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内毒素水平:
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来源:
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表达方式:
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Accession #:
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CAS号:
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分子式:
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溶解度:
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PubChem CID: