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产品名称:
HSC70 Protein
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别名:
Constitutive heat shock protein 70 Protein, HSC54 Protein, HSC71 Protein, HSC73 Protein, HSP71 Protein, HSP74 Protein, HSPA10 Protein, HSPA8 Protein, LAP1 Protein, NIP71 Protein
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产品描述:
Active Human Recombinant HSC70 Protein
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反应种属:
Human
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宿主来源:
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实验应用:
WB | SDS-PAGE | ATPase Activity Assay | Functional Assay | ELISA
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靶标/特异性:
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同种型:
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推荐稀释度:
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免疫原:
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免疫原种属:
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克隆性:
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克隆号:
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纯化方式:
Affinity Purified
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偶联:
His tag
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产品浓度:
Lot/batch specific. See included datasheet.
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表达系统:
E. coli
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阳性对照:
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保存温度:
-20ºC
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运输温度:
Blue Ice or 4ºC
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细胞定位:
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预期分子量:
~70 kDa
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产品形式:
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存储溶液:
20mM Tris/HCl pH7.5, 1mM EDTA
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有效期:
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产地:
加拿大
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外观:
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生产商:
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功能与背景:
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
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URL:
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灵敏度:
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检测范围:
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检测方法:
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样本类型:
20mM Tris/HCl pH7.5, 1mM EDTA
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产品特点:
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纯度:
>90%
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生物活性:
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内毒素水平:
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来源:
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表达方式:
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Accession #:
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CAS号:
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分子式:
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溶解度:
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PubChem CID: