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产品名称:
Rab5 Protein
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别名:
Rab 5A Protein, RAS associated protein RAB5A Protein, Ras related protein Rab 5 A Protein
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产品描述:
Human Recombinant Rab5 Protein
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反应种属:
Human
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宿主来源:
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实验应用:
WB | SDS-PAGE
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靶标/特异性:
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同种型:
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推荐稀释度:
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免疫原:
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免疫原种属:
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克隆性:
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克隆号:
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纯化方式:
Affinity Purified
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偶联:
His tag
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产品浓度:
Lot/batch specific. See included datasheet.
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表达系统:
E. coli
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阳性对照:
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保存温度:
-20ºC
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运输温度:
Blue Ice or 4ºC
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细胞定位:
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预期分子量:
~26 kDa
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产品形式:
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存储溶液:
20mM Tris/HCl, pH7.5, 0.15M NaCl
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有效期:
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产地:
加拿大
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外观:
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生产商:
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功能与背景:
Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
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URL:
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灵敏度:
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检测范围:
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检测方法:
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样本类型:
20mM Tris/HCl, pH7.5, 0.15M NaCl
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产品特点:
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纯度:
>90%
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生物活性:
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内毒素水平:
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来源:
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表达方式:
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Accession #:
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CAS号:
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分子式:
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溶解度:
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PubChem CID: