Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml/长柔毛野豌豆外源凝集素，琼脂糖交联

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品牌： Vectorlabs

产品货号： AL-1233-2

规格： 2 ml

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折后价：

数量： - +

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产品名称:

Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml/长柔毛野豌豆外源凝集素，琼脂糖交联
别名:

长柔毛野豌豆外源凝集素，琼脂糖交联
产品描述:

Vicia villosa lectin VVL, VVA lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.Agarose bound* Vicia villosa lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine*3 mg lectin/ml gel
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实验应用:

Glycobiology,Affinity Chromatography
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推荐稀释度:

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactosamine can be used.Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
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Agarose
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保存温度:

2° - 8°C – Do Not Freeze
运输温度:

Ambient
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存储溶液:

10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide
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产地:

美国
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10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide
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