产品描述:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate (Item No. 17865) to 1,3-bisphosphoglycerate during glycolysis and is involved in numerous additional cellular processes, including intracellular trafficking, receptor-mediated signaling, apoptosis, DNA repair, and the oxidative stress response.{54459,54460} It exists as a tetramer and is composed of an N-terminal domain, which contains binding sites for NAD+, phosphatidylserine, RNA, and glutathione, and a C-terminal catalytic domain.{54461} GAPDH is widely expressed and primarily localizes to the cytosol, where it has roles in glycolysis and intracellular trafficking.{54459,60077} It also localizes to the nucleus, mediating DNA integrity, gene transcription, and apoptosis, as well as to cellular membranes, where it has roles in membrane fusion and iron transport.{60077} GAPDH expression is increased by insulin, hypoxia-inducible factor-1 (HIF-1), p53, and nitric oxide (NO) and decreased by acetylated histones.{54459,54462} Aberrant mRNA and protein levels of GAPDH have been found in tumor biopsies from patients with a variety of cancers, including lung, renal cell, colorectal, or breast cancer.{60078} Cayman's GAPDH (human, recombinant) protein consists of 346 amino acids and has a calculated molecular weight of 38 kDa.