产品描述:
VU534 is an activator of N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD), which hydrolyzes NAPEs into N-acyl ethanolamines (NAEs) and phosphatidic acid.{49868} It increases NAPE-PLD activity in cell-free assays (EC50s = 0.3 and 0.93 µM for the recombinant mouse and human enzymes, respectively). VU534 (20 µM) also increases NAPE-PLD activity in RAW 264.7 cells, an effect that can be blocked by the NAPE-PLD inhibitor bithionol (Item No. 21844), and in HepG2 cells (EC50 = 1.5 µM). It also inhibits soluble epoxide hydrolase (sEH) with a maximal inhibition of 55% and weakly inhibits fatty acid amide hydrolase (FAAH). VU534 (10 µM) increases efferocytosis in isolated wild-type mouse bone marrow-derived macrophages (BMDMs) but not in BMDMs isolated from Napepld-/- mice.