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CUTANA™ Nuclei Extraction Buffer
品牌： EpiCypher
产品货号： 21-1026
规格： 100 Reactions

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  • 产品名称:
    CUTANA™ Nuclei Extraction Buffer
  • 别名:
  • 产品描述:
    CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer: mouse NIH3T3 fibroblast cells human A549 non-small cell lung cancer (NSCLC) cells human K562 leukemia cells human NCI-H1299 non-small cell lung cancer (NSCLS cells) human bone marrow derived macrophages human TIG-1 fetal lung cells human monocyte derived macrophages human LoVo colorectal cancer cells human MV-4-11 macrophage cells human LNCaP prostate carcinoma cells human SUM149 triple negative breast cancer (TNBC) cells human renal primary cells human GM24385 B-lymphocyte (aka HG002) cells human peripheral blood mononuclear cells (PBMCs) human MCF7 breast cancer cells human intestinal tissue human MDA-MB-231 breast cancer cells human HEPM embryonic cells human SK-MEL-2 melanoma cells The reagent is prepared by supplementing Pre-Nuclei Extraction Buffer with protease inhibitor and spermidine fresh on the day of use. Utilize this buffer in EpiCypher’s CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag (epicypher.com/protocols) to obtain the highest quality nuclei for your genomic mapping assay.
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  • 实验应用:
    CUT&RUN, CUT&Tag
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  • 保存温度:
    Store the Pre-Nuclei Extraction Buffer at 4ºC and the 1 M Spermidine at -20ºC. Components are stable for 6 months upon date of receipt.
  • 运输温度:
    DO NOT FREEZE!! Frozen cold packs.
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  • 产品形式:
    Item CAT CUTANA™ Pre-Nuclei Extraction Buffer 21-1026a 1 M Spermidine 21-1026b
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  • 产地:
    美国
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