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产品名称:
TMB dihydrochloride
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别名:
3,3′,5,5′-Tetramethylbenzidine dihydrochloride; TMBZ
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产品描述:
TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (lambdamax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
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反应种属:
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宿主来源:
Synthetic
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实验应用:
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同种型:
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免疫原:
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产品浓度:
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表达系统:
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阳性对照:
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保存温度:
+4°C
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运输温度:
Ambient
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细胞定位:
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预期分子量:
240.3 . 72.9
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产品形式:
solid
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存储溶液:
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有效期:
Stable for at least 2 years after receipt when stored at +4°C.
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产地:
瑞士
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产品特点:
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纯度:
>95% (HPLC)
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来源:
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Accession #:
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CAS号:
64285-73-0
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分子式:
C16H20N2 . 2HCl
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溶解度:
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PubChem CID: