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产品名称:
BCECF-AM Solution
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别名:
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid
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产品描述:
Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: lambdaex 490nm; lambdaem 535nm; lambdaex=505nm; lambdaem=520nm in DMSO.
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反应种属:
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宿主来源:
Synthetic.
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同种型:
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产品浓度:
5mM in DMSO (1mg in 0.25ml DMSO)
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表达系统:
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阳性对照:
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保存温度:
-20°C
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运输温度:
BLUE ICE
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细胞定位:
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预期分子量:
808.7
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产品形式:
liquid
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存储溶液:
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有效期:
Stable for at least 2 years after receipt when stored at -20°C.
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产地:
瑞士
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纯度:
>95% (HPCE)
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Accession #:
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CAS号:
117464-70-7
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分子式:
C42H40O21
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溶解度:
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PubChem CID: