产品描述:
TNP-GTP is a fluorescent derivative of the energy substrate for protein synthesis and gluconeogenesis, guanosine 5'-triphosphate (GTP; Item No. 16060).{67412} It displays an emission maximum of 552 nm upon excitation at 410 nm in water, with fluorescence increasing in intensity and shifting to 544 and 532 nm in 40 and 80% N,N-dimethylformamide, respectively, which is less polar than water.{67412} TNP-GTP is an inhibitor of glutamate dehydrogenase (Ki = 2.7 μM). When bound to glutamate dehydrogenase, the fluorescence intensity of TNP-GTP is increased and the emission shifts from 552 to 545 nm, an effect that can be blocked by addition of GTP. TNP-GTP is an antagonist of the purinergic P2X2 and P2X2/3 receptors (IC50s = 0.4 and 1.2 nM, respectively).{36447} It also selectively inhibits rat soluble guanylyl cyclase (sGC; Ki = 11 nM) over bovine liver glutamate dehydrogenase (GDH; Ki = 2.7 µM) and the calmodulin-dependent B. pertussis adenylyl cyclase (AC) toxin (Kis = 20 and 320 µM in the presence of manganese or magnesium, respectively).{67413,67412,65691}