产品描述:
Coelenterazine (Item No. 16123) is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.{22804} Coelenterazine can be used to reconstitute the aequorin complex both in vivo and in vitro, emitting blue light when bound to calcium ions. Coelenterazine h is a synthetic derivative of native coelenterazine that exhibits 16-fold higher luminescence intensity (emission maximum ~466 nm; half-total time of 0.6-1.2 sec) than native coelenterazine.{6107} Aequorin complexes reconstituted with coelenterazine h are reported to be more sensitive to calcium ions than those employing the native constituent, providing a useful indicator for small changes in Ca2+ concentrations.{27442}