VectaCell Trolox Antifade Reagent for Live Cell Imaging, 2 ml/活细胞成像防淬灭试剂

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品牌： Vectorlabs

产品货号： CB-1000-2

规格： 2 ml

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数量： - +

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产品名称:

VectaCell Trolox Antifade Reagent for Live Cell Imaging, 2 ml/活细胞成像防淬灭试剂
别名:

活细胞成像防淬灭试剂
产品描述:

VectaCell Trolox Antifade Reagent for Live Cell Imaging is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E, that efficiently prevents formation of different reactive oxygen species, such as singlet oxygen (1O2), superoxide anion (O2-) or hydrogen peroxide (H2O2).VectaCell™ Trolox contains both Trolox and its oxidized form Trolox-quinone. This redox system reduces photo-bleaching and blinking during live cell imaging. 1, 2 Trolox has been shown to have a cytoprotective effect and low cytotoxicity for different cell lines.3Photo-excitation of a fluorophore generates reactive oxygen species that can lead to photo-bleaching as well as oxidative damage in cells. Although reduction of the light intensity usually decreases fading, it also reduces the sensitivity of the assay.Trolox has been used to reduce fluorophore bleaching1 as well as the fluorophore blinking2 during the observation of fluorescent labels. Although the mechanism is not completely understood it is attributed to the combination of its oxygen-scavenger capacity that prevents oxidative bleaching and the complementary reduction reaction that helps prevent blinking.
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Immunofluorescence,Cellular Imaging
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Instructions for use: Dilute the Trolox Solution into culture media or imaging buffer to a final concentration between 0.1 mM and 1 mM. Notes: The optimal working concentration of Trolox in imaging media will depend on the cell type and their tolerance to hypoxia. For cells that have low tolerance to hypoxia, or can be affected by the addition of ethanol, the appropriate controls should be included in the assay.
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100 mM (in ethanol)
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2° - 8°C – Do Not Freeze
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Ambient
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美国
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