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EpiDyne®-FRET Nucleosome Remodeling Assay Substrate
品牌: EpiCypher
产品货号: 16-4201
规格: 50 µg

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数量: - +
产品详情
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  • 产品名称:
    EpiDyne®-FRET Nucleosome Remodeling Assay Substrate
  • 别名:
  • 产品描述:
    This product provides a high throughput fluorescent readout of nucleosome remodeling when paired with enzymatically active remodeling complexes, such as SMARCA4, SMARCA2, and ACF (EpiCypher 15-1014, 15-1015, 15-1013). The EpiDyne®-FRET mononucleosome is assembled from recombinant human histones expressed in E. coli (two each of histones H2A-Cy5, H2B, H3.2 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped with a 212 base-pair DNA template bearing a 5’ Cy3, the Widom 601 nucleosome positioning element [1], and a DpnII restriction enzyme motif (GATC) that is exposed upon remodeling. H2A has a Thr to Cys substitution at residue 120, where Cy5 is conjugated. H3.2 has a Cys to Ala substitution at residue 110.
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  • 保存温度:
    Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
  • 运输温度:
    Dry ice.
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  • 产品形式:
    Purified recombinant mononucleosomes, (21.8 µg protein weight, 50 µg DNA+protein) in 44.2 µL of 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 240,650 Da. Molarity = 4.7 µM.
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  • 产地:
    美国
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