Prostaglandin E2 TR-FRET Assay Kit-其他特色试剂盒-ELISA_抗体_蛋白_核苷酸

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Prostaglandin E2 TR-FRET Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：702200

规格：384 wells

目录价：￥8036

产品详情

产品名称:

Prostaglandin E2 TR-FRET Assay Kit

别名:

Dinoprostone TR-FRET|PGE2 TR-FRET

产品描述:

Cayman’s Prostaglandin E2 (PGE2) TR-FRET Assay Kit provides a robust and easy-to-use time-resolved Förster resonance energy transfer (TR-FRET) platform for measurement of PGE2 in cell culture supernatants. The assay utilizes a PGE2 monoclonal antibody directly labeled with a europium (Eu3+) chelate as a donor molecule and fluorescently labeled PGE2 as the acceptor molecule. In the absence of free PGE2, the acceptor-labeled PGE2 is bound to the europium-labeled PGE2 antibody resulting in FRET from the donor to the acceptor upon excitation of the Eu3+ chelate at 340 nm and an emission from the acceptor at 665 nm. If free PGE2 is present, it will compete with the PGE2 acceptor for binding to the donor-labeled PGE2 antibody, causing a decrease in the TR-FRET signal. The specific signal at 665 nm will be inversely proportional to the PGE2 concentration in the sample. Data can be expressed as either the signal at 665 nm or the 665/615 nm ratio. This assay has a range of 0.038-600 ng/ml with lower limit of detection of approximately 0.16 ng/ml.

保存温度:

-20

运输温度:

-20

有效期:

365

来源:

Animal/Mouse|Animal/Bovine

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