货号:702200 | 规格:384 wells | 目录价:¥8036 |
产品详情
产品名称:
Prostaglandin E2 TR-FRET Assay Kit
别名:
Dinoprostone TR-FRET|PGE2 TR-FRET
产品描述:
Cayman’s Prostaglandin E2 (PGE2) TR-FRET Assay Kit provides a robust and easy-to-use time-resolved Förster resonance energy transfer (TR-FRET) platform for measurement of PGE2 in cell culture supernatants. The assay utilizes a PGE2 monoclonal antibody directly labeled with a europium (Eu3+) chelate as a donor molecule and fluorescently labeled PGE2 as the acceptor molecule. In the absence of free PGE2, the acceptor-labeled PGE2 is bound to the europium-labeled PGE2 antibody resulting in FRET from the donor to the acceptor upon excitation of the Eu3+ chelate at 340 nm and an emission from the acceptor at 665 nm. If free PGE2 is present, it will compete with the PGE2 acceptor for binding to the donor-labeled PGE2 antibody, causing a decrease in the TR-FRET signal. The specific signal at 665 nm will be inversely proportional to the PGE2 concentration in the sample. Data can be expressed as either the signal at 665 nm or the 665/615 nm ratio. This assay has a range of 0.038-600 ng/ml with lower limit of detection of approximately 0.16 ng/ml.
保存温度:
-20
运输温度:
-20
有效期:
365
来源:
Animal/Mouse|Animal/Bovine
您可能感兴趣的产品
SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3)
货号:GTX537371-23
AMP+ MaxSpec® Kit
货号:710000
8-Isoprostane Affinity Purification Kit
货号:501110
THUNDER™ Mouse IL-2 TR-FRET Biomarker Assay Kit
货号:500311
THUNDER™ Mouse IFNβ TR-FRET Biomarker Assay Kit
货号:500309
THUNDER™ Mouse IFNγ TR-FRET Biomarker Assay Kit
货号:500308
THUNDER™ Human GM-CSF TR-FRET Biomarker Assay Kit
货号:500307
THUNDER™ Human CCL4/MIP-1β TR-FRET Biomarker Assay Kit
货号:500306
促销活动
相关产品推荐
AMP+ MaxSpec® Kit
货号:710000
THUNDER™ Human IL-10 TR-FRET Biomarker Assay Kit
货号:500302
THUNDER™ Human IL-12 p70 TR-FRET Biomarker Assay Kit
货号:500303
THUNDER™ Human IL-18 TR-FRET Biomarker Assay Kit
货号:500304
THUNDER™ Human Insulin TR-FRET Biomarker Assay Kit
货号:500305
THUNDER™ Human CCL4/MIP-1β TR-FRET Biomarker Assay Kit
货号:500306