SIRT1 FRET-Based Screening Assay Kit-抑制剂筛选试剂盒-ELISA_抗体_蛋白_核苷酸

首页产品中心抑制剂筛选试剂盒

SIRT1 FRET-Based Screening Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：10010991

规格：96 wells

目录价：￥4872

产品详情

产品名称:

SIRT1 FRET-Based Screening Assay Kit

产品描述:

Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs). Human SIRT1 is the homolog of yeast Sir2 (Silent information regulator 2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Small molecule activators of SIRT1, such as resveratrol, extend lifespan in yeast and C. elegans in a manner that resembles caloric restriction. Cayman’s SIRT1 FRET-based Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence can be easily analyzed using an excitation wavelength of 335-345 nm and emission wavelength of 440-465 nm.

保存温度:

-80

运输温度:

-80

有效期:

365

您可能感兴趣的产品

SARS-CoV-2 Inhibitor Screening Kit

货号：AG-48B-0001-KI01

15-hydroxy Prostaglandin Dehydrogenase Inhibitor Screening Kit

货号：702180

ENPP1 Fluorescent Inhibitor Screening Assay Kit