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折扣/货期| 货号：SA-5001-1 | 规格：1 mg | 目录价：￥2820 |
产品详情
产品名称:
Streptavidin, Fluorescein, 1 mg
产品描述:
Fluorescein Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.Streptavidin, fluorescein labeled, has an excitation maximum at 495 nm and an emission maximum at about 515 nm. This product has an optimal fluorochrome to protein ratio and is one of the reagents we recommend for routine immunofluorescence applications. Compared to conventional primary and secondary fluorescent techniques, Fluorescein Streptavidin can provide greater sensitivity and lower background staining.
实验应用:
Immunofluorescence,In situ hybridization,Flow Cytometry/Cell Separation
推荐稀释度:
For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate-
buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other
biotin-containing solutions as diluents.Serum also can contain biotin and should
not be added to diluents.
The recommended range of concentrations for staining of cells or tissues is
5-30µg/ml.
偶联:
Fluorescein
产品浓度:
1.0 mg active conjugate/ml
保存温度:
2° - 8°C – Do Not Freeze
运输温度:
Ambient
产品形式:
Concentrate
存储溶液:
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
产地:
美国
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