THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit-时间分辨荧光分析试剂盒-ELISA_抗体_蛋白_核苷酸

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THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：500246-2400 wells	规格：2400 wells	目录价：￥79472
货号：500246-480 wells	规格：480 wells	目录价：￥18736
货号：500246-4800 wells	规格：4800 wells	目录价：￥98784
货号：500246-96 wells	规格：96 wells	目录价：￥5968
货号：500246	规格：9600 wells	目录价：￥122896

产品详情

产品名称:

THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit

别名:

Eukaryotic Translation Initiation Factor 4E|mRNA Cap-binding Protein

产品描述:

The Phospho-eIF4E (S209) assay kit is a homogeneous time_x0002_resolved Förster resonance energy transfer (TR-FRET) sandwich immunoassay. The THUNDER™ Cell Signaling assay workflow consists of 3 steps. Following cell treatment, cells are first lysed with the specific Lysis Buffer provided in the kit. Then Phospho-eIF4E (S209) in the cell lysates is detected with a pair of fluorophore-labeled antibodies in a simple "add-incubate_x0002_measure" format (single-step reagent addition; no wash steps). One antibody is labeled with a donor fluorophore (Europium chelate; Eu-Ab1) and the second with a far-red acceptor fluorophore (FR_x0002_Ab2). The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two dyes into close proximity. Excitation of the donor Europium chelate molecules with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a FRET from the donor to the acceptor molecules, which in turn emit a TR-FRET signal at 665 nm. Residual energy from the Eu chelate generates light at 615 nm. The signal at 665 nm is proportional to the concentration of Phospho-eIF4E (S209) in the cell lysate. Data can be expressed as either the signal at 665 nm or the 665 nm/615 nm ratio.

保存温度:

-80

运输温度:

-80

有效期:

180

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