Lightning Link 系列金属标记试剂盒

2021-05-11

浏览次数： 58

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Expedeon 2019 年新产品 Lightning Link 系列金属标记试剂盒现在有多达30种啦！

手工操作只需30秒！
1 小时内完成标记
无繁琐的分离步骤，100% 抗体回收率
温和化学反应，标记物稳定
可选金属多，以便多路测定
批间重复性好
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操作步骤

Lightning Link 系列金属标记试剂盒

可选标记

Lightning-Link^® 金属标记试剂盒
Lightning-Link^® 139La	Lightning-Link^® 159Tb
Lightning-Link^® 141Pr	Lightning-Link^® 160Gd
Lightning Link^®-142Nd	Lightning-Link^® 162Dy
Lightning-Link^® 143Nd	Lightning-Link^® 164Dy
Lightning-Link^® 144Nd	Lightning-Link^® 165Ho
Lightning-Link^® 146Nd	Lightning Link^®-166Er
Lightning-Link^® 147Sm	Lightning Link^®-167Er
Lightning-Link^® 148Nd	Lightning-Link^® 168Er
Lightning-Link^® 149Sm	Lightning-Link^®169Tm
Lightning-Link^® 151Eu	Lightning-Link^® 170Er
Lightning-Link^® 152Sm	Lightning-Link^® 171Yb
Lightning-Link^® 153Eu	Lightning-Link^® 172Yb
Lightning-Link^® 154Sm	Lightning-Link^® 174Yb
Lightning Link^®-156Gd	Lightning-Link^® 175Lu
Lightning-Link^® 158Gd	Lightning-Link^® 176Yb

实验数据

Lightning Link 系列金属标记试剂盒

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link^® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Lightning Link 系列金属标记试剂盒

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link^® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Lightning Link 系列金属标记试剂盒