● 在流式平台上计数细胞
● 利用抗体标记细胞并利用123count eBeads计算该细胞群数量
● 可同时并用FVD细胞存活染料标记活细胞计数
123count eBeads™应用领域
● 全血细胞计数
● 流式细胞仪计数功能验证
● 精子浓度计数
● 可并用细胞存活染料7-AAD, PI或FVD计数细胞存活率
● 使用磁珠细胞分选试剂盒后计算细胞分选产率与纯度
货号:01-1234-42 , 100个反应
123count eBeads™使用方式
● 加入123count eBeadsTM→选定待计数的细胞 →公式计算细胞浓度。
● 9加入*已知浓度的123count eBeads 进未知浓度的细胞样本
● 流式细胞仪同时读取细胞与123count eBeads
● 公式计算细胞浓度如下:
Whole blood was stained with CD45 PE, CD3 FITC and CD4 APC, and then
lysed with 1-step Fix/Lyse Solution (10X)(cat. no. 00-5333). 123count
eBeads™ were added after lysis. Lymphocytes were gated on CD45+ cells,
and then analyzed for CD3+ and CD4+ cells (left). 123count eBeads were
counted based on bright fluorescence in FITC and PerCP-eFluor® 710 (right).
全血CD4实际细胞计数示范
= (流式细胞仪显示细胞数量/流式细胞仪显示eBead数量) X eBead标示浓度
= (541个细胞/1009 eBeads) X 998 eBeads/ul
= 535个细胞/ul
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