关于BrdU
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU)，中文名为5-溴脱氧尿嘧啶核苷，是一种合成的核苷，胸腺嘧啶(thymidine)的类似物。BrdU普遍使用于检测活组织中的增殖细胞，对研究细胞动力学有重要意义。
BrdU可被正在复制的细胞(S期)所摄取，整合至新合成的DNA链中，可取代胸腺嘧啶(thymidine)。然后可用BrdU特异性的抗体来检测被摄入的BrdU，以此来反应细胞的增殖。DNA须经过变性(denaturation)才可与抗体结合，通常会用酸或热来处理细胞。
工欲善其事，必先利其器，BrdU检测工具，您了解多少？
BrdU细胞增殖ELISA检测试剂盒
Exalpha公司研发的Frontier™ BrdU 细胞增殖检测分析试剂盒，是一种完全适用于高通量应用、非同位素标记法的ELISA检测试剂盒(可提供显色法和化学发光法)，可检测掺入到贴壁细胞及非贴壁细胞新合成DNA链中的BrdU。
试剂盒检测原理
主要以细胞层作为固相载体, 将BrdU摄入微孔板中培养的细胞。在细胞培养的最后2-14小时，把BrdU加入微孔板中，BrdU将被掺入到分裂细胞的DNA中。为确保抗体与BrdU有效结合，需要将细胞固定并做破膜处理，对DNA进行变性。所有操作仅需通过一步固定剂(Fixing solution)处理即可。将BrdUd单克隆抗体加入到板孔中，孵育1小时，未结合的抗体通过洗涤步骤去除，然后加入HRP标记的羊抗小鼠二抗，最后加入HRP底物显色。
| 名称 | 货号 | 规格 | 品牌 |
Frontier™ Bromodeoxyuridine (BrdU)
Cell Proliferation ELISA Kit(比色法) | X1327K1 | 200 tests | Exalpha |
Frontier™ Bromodeoxyuridine (BrdU)
Cell Proliferation ELISA Kit(比色法) | X1327K2 | 1000 tests | Exalpha |
Frontier™ Bromodeoxyuridine (BrdU)
Cell Proliferation ELISA Kit(比色法) | X1327K3 | 5000 tests | Exalpha |
Frontier™ Chemiluminescent
Bromodeoxyuridine (BrdU) Cell
Proliferation ELISA Kit（化学发光法） | X1623K1 | 200 tests | Exalpha |
Frontier™ Chemiluminescent
Bromodeoxyuridine (BrdU) Cell
Proliferation ELISA Kit（化学发光法） | X1623K2 | 1000 tests | Exalpha |
Frontier™ Chemiluminescent
Bromodeoxyuridine (BrdU) Cell
Proliferation ELISA Kit（化学发光法） | X1623K3 | 5000 tests | Exalpha |
BrdU免疫组化检测试剂盒
BrdU被掺入到分裂细胞的DNA中，然后加生物素化的BrdU特异性抗体孵育，再通过HRP-链亲和素结合物催化DAB底物显色。该试剂盒可用对任何种属的组织进行染色。
| 名称 | 货号 | 规格 | 品牌 |
| Frontier™ Bromodeoxyuridine (BrdU) Immunohistochemistry Kit | X1545K | 50 slides | Exalpha |
BrdU流式染色试剂盒
| 名称 | 应用 | 货号 | 品牌 |
| BrdU Staining Buffer Set | FC | 00-5525 | eBioscience |
| BrdU Staining Kit for Flow Cytometry APC | FC | 8817-6600 | eBioscience |
| BrdU Staining Kit for Flow Cytometry eFluor® 450 | FC | 8848-6600 | eBioscience |
| BrdU Staining Kit for Flow Cytometry FITC | FC | 8811-6600 | eBioscience |
| BrdU Staining Kit for Flow Cytometry PE | FC | 8812-6600 | eBioscience |
| BrdU Staining Kit for Flow Cytometry PerCP-eFluor® 710 | FC | 8846-6600 | eBioscience |
BrdU抗体
| 名称 | 货号 | 规格 | 品牌 |
| Sheep anti BrdU Polyclonal Antibody | A205P | 250 µg | Exalpha |
| Mouse Anti-Bromodeoxyuridine Monoclonal Antibody | X1028 | 100 µg | Exalpha |
| Mouse Anti-Bromodeoxyuridine Monoclonal Antibody(FITC conjugate) | X1543M | 100 µg | Exalpha |
| RAT ANTI BrdU | MCA2060 | 0.2 mg | AbD Serotec |
| RAT ANTI BrdU:Alexa Fluor® 488 | MCA2060A488 | 100tests | AbD Serotec |
| RAT ANTI BrdU:Biotin | MCA2060B | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU:FITC | MCA2060F | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU:FITC | MCA2060FT | 0.5 ml | AbD Serotec |
| RAT ANTI BrdU | MCA2060GA | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU:HRP | MCA2060P | 0.5 mg | AbD Serotec |
| RAT ANTI BrdU:HRP | MCA2060PA | 0.1 mg | AbD Serotec |
| RAT ANTI BrdU | MCA2060T | 20 µg | AbD Serotec |
| MOUSE ANTI BrdU | MCA2483 | 0.2 mg | AbD Serotec |
| MOUSE ANTI BrdU:FITC | MCA2483FA | 50 µg | AbD Serotec |
| MOUSE ANTI BrdU | MCA2483T | 20 µg | AbD Serotec |
| RAT ANTI BrdU | OBT0030 | 0.5 mg | AbD Serotec |
| RAT ANTI BrdU | OBT0030CX | 0.25 ml | AbD Serotec |
| RAT ANTI BrdU:FITC | OBT0030F | 1 ml | AbD Serotec |
| RAT ANTI BrdU | OBT0030G | 0.25 mg | AbD Serotec |
| RAT ANTI BrdU | OBT0030S | 5 ml | AbD Serotec |
| BrdU [MoBu-1] antibody (Biotin) | GTX10362 | 100μg | GeneTex |
| BrdU antibody [BU6-4] | GTX12219 | 50μg | GeneTex |
| BrdU antibody | GTX128091 | 100μl | GeneTex |
| BrdU antibody [BU1/75 (ICR1)] | GTX26326 | 100μg | GeneTex |
| BrdU antibody [BU1/75 (ICR1)] (FITC) | GTX27384 | 250μl | GeneTex |
| BrdU antibody [B33.1] | GTX27781 | 500μl | GeneTex |
| BrdU antibody [B684M] | GTX44314 | 100μg | GeneTex |
| BrdU antibody [IIB5] (FITC) | GTX50089 | 500μl | GeneTex |
| BrdU, mAb (2B1) | ADI-MSA-200-E | 1*100ug | ENZO |
| 5-Bromodeoxyuridine, mAb (PRB-1) (biotin conjugate) | ALX-804-197B-T060 | 60 tests | ENZO |
| 5-Bromodeoxyuridine, mAb (PRB-1) (fluorescein conjugate) | ALX-804-197L-T060 | 60 tests | ENZO |
| Anti-BrdU APC | 17-5071 | 25/100tests | eBioscience |
| Anti-BrdU eFluor® 450 | 48-5071 | 25/100tests | eBioscience |
| Anti-BrdU FITC | 11-5071 | 25/100tests | eBioscience |
| Anti-BrdU PE | 12-5071 | 25/100tests | eBioscience |
| Anti-BrdU PerCP-eFluor® 710 | 46-5071 | 25/100tests | eBioscience |
| Anti-BrdU Purified | 14-5071 | 25/100ug | eBioscience |
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