品牌：Mirus

货号：MIR 7021

品名：Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

产品信息：

产品类型：核酸定位试剂盒

规格：每个试剂盒包含Label IT®Tracker™试剂，Label IT®重组液，和10X标记缓冲液。

储存条件：-20℃

保质期：1年（重组后6个月）。

产品介绍：

特点：

多功能标签-高效标签和可视化的DNA。

一步化学法-易于和一致地控制标记反应。

高效标记-细胞中DNA的最佳可视化。

卓越追踪-在转染标记(质粒)DNA后监测亚细胞定位和报告转基因表达。

相关数据：

Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.

Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.

更多详情请咨询Mirus中国授权代理商-欣博盛生物

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