雌二醇(estradiol) 是由卵巢内卵泡的颗粒细胞分泌的类固醇激素,是主要的雌激素,负责调节女性特征、附属性器官的成熟和月经-排卵周期,促进乳腺导管系统的产生,有α,β两种类型。其代谢物是雌酮及雌三醇。雌二醇的靶器官为子宫、阴道、输卵管和垂体。雌激素能促使细胞合成DNA、RNA和相应组织内各种不同的蛋白质。β-雌二醇(17β-Estradiol)可引起促性腺激素的产生从而促进排卵。
ENZO 17β-Estradiol ELISA kit是一种比色竞争法酶免疫检测试剂盒,3小时内可以得到检测结果。在405nm处读取吸光度。
● 特异性强,与相关类固醇交叉反应性低
● 安全无放射性的酶法检测
● 重复性好
● 简单易用的颜色编码试剂最大限度地减少错误
● 超越半定量Western Blot分析的全定量检测
产品信息
产品货号 | ADI-900-008/ ADI-901-008 |
产品名称 | 17β-Estradiol ELISA kit(17β-雌二醇ELISA试剂盒) |
规格 | 96 wells/ 5x96 wells |
其他名称 | Estrogen |
灵敏度 | 28.5 pg/ml |
检测范围 | 29.3-30,000 pg/ml |
检测时间 | <3 hours |
样本种属 | Species independent |
样本类型 | culture supernatants and saliva from any species. For serum and plasma use ADI-900-174 |
保存温度 | +4°C |
试剂盒组分 | GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution n |
部分产品引用文献
1. Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022)
2. A novel method to differentiate tonsil-derived mesenchymal stem cells in vitro into estrogen-secreting cells: H. Kim, et al.; Tissue Eng. Regen. Med. 18, 253 (2021), Application(s): ELISA using culture supernatants
3. Chronic irisin exposure decreases sexual incentive motivation in female rats: N. Ulker, et al.; Physiol. Behav. 232, 113341 (2021), Application(s): Rat serum
4. Hormone autocrination by vascularized hydrogel delivery of ovary spheroids to rescue ovarian dysfunctions: H.J. Yoon, et al.; Sci. Adv. 7, eabe8873 (2021)
5. Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats: L.C. Santos, et al.; Biol. Reprod. 61, 1093 (2021)
6. Neonatal androgenization in rats affects oocyte maturation: G. Anesetti & R. Chávez-Genaro; Reprod Sci. 28, 2799 (2021)
7. Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021)
8. Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants
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