最近的研究表明，维生素D水平影响各种疾病状态，并与人类健康的许多指标相关联。这些疾病包括骨质疏松症和关节炎等骨骼疾病，也包括高血压、糖尿病、癌症和心脏病等其他疾病。Enzo Life Sciences的25-羟基维生素D ELISA试剂盒为快速便捷检测人血浆和血清中25-羟基维生素D水平提供了一种方法。
维生素D向活性形式的转化始于7-脱氢胆固醇被紫外线作用形成母体维生素D3。或者来源于食物摄取的母体维生素D2。这些母体化合物被运输到肝脏并羟基化为25(OH)维生素D。这种代谢物随后被运输到肾脏，再次羟基化为1，25(OH)2维生素D，这是维生素D2的生物活性形式。维生素D代谢物的水平随着母体维生素D摄取量的增加而成比例增加。与活性形式相比，25(OH)维生素D在循环中的半衰期和稳定性更长，这是25(OH)维生素D代谢物的检测被用作总维生素D浓度指标的原因。
产品信息
产品货号 | ADI-900-215-0001 |
产品名称 | 25(OH) Vitamin D ELISA kit |
规格 | 96 wells |
灵敏度 | 1.98 ng/ml |
检测范围 | 0.5-1010 ng/ml |
其他名称 | 25-Hydroxyvitamin D |
检测时间 | ~1.5 hours |
适用样本类型 | 血清，血浆 |
适用种属 | 人 |
交叉反应 | 25(OH) Vitamin D3 (100%), 25(OH) Vitamin D2 (~80%), 1,25(OH)2-Vit. D2 (>100%), 1,25(OH)2-Vitamin D3 (>100%), 3-epi 25(OH) Vitamin D3 (~10%), 24,25(OH)2-Vitamin D3 (~6%) and <1%: 1α(OH) Vitamin D2, 1α(OH) Vitamin D3, Vitamin D2, Vitamin D3 |
试剂盒组分 | Microtiter Plate, Conjugate Concentrate, Antibody, Dissociation Buffer, Conjugate Diluent, Sample Diluent, Wash Buffer Concentrate, Standards, Substrate, Stop Solution, Assay Layout |
标准曲线示例
部分产品文献引用
1. Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021)
2. Vitamin K and D Status in Patients with Knee Osteoarthritis: An Analytical Cross-sectional Study: A. Askari, et al.; Mediterr. J. Rheumatol. 32, 350 (2021)
3. Compounding effect of vitamin D3 diet, supplementation, and alcohol exposure on macrophage response to mycobacterium infection: M.E. Gough, et al.; Tuberculosis (Edinb.) 116S, S42 (2019), Application(s): ELISA using culture supernatants
4. VDR gene methylation as a molecular adaption to light exposure: Historic, recent and genetic influences: E.L. Beckett, et al.; Am. J. Hum. Biol. 29, (2017)
5. A study of Vitamin D Levels in hypothyroid patients, a case control study in a tertiary care hospital of central India: R. Kohokare, et al.; NJMDR 4, 89 (2016), Application(s): Vitamin D levels were measured from serum of hypothyroid patients
6. Evaluation of vitamin D relationship with type 2 diabetes and systolic blood pressure: S.B. Nayak, et al.; BMJ Open Diabetes Res. Care 4, e000285 (2016), Application(s): Determination of 25(OH)D levels in human plasma and whole blood samples
7. Reduced 25-OH vitamin D in patients with autoimmune cytopenias, clinical correlations and literature review: B. Fattizzo, et al.; Autoimmun. Rev. 15, 770 (2016), Application(s): Measured Vitamin D in 40 year old humans
8. Relationship between methylation status of vitamin D-related genes, vitamin D levels, and methyl-donor biochemistry: E.L. Beckett, et al.; J. Nutr. Int. Metab. 6, 8 (2016), Application(s): ELISA using human plasma,
9. Vitamin D deficiency is associated with endothelial dysfunction and increases type I interferon gene expression in a murine model of systemic lupus erythematosus: J.A. Reynolds, et al.; Arthritis Rheumatol. 68, 2929 (2016)
10. Vitamin D status in different stages of disease severity in dogs with chronic valvular heart disease: T. Osuga, et al.; J. Vet. Intern. Med. 29, 1518 (2015), Application(s): ELISA using dog serum
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