BioXCell热销产品--RecombiMAb anti-mouse CSF1R (CD115)
产品描述：
AFS98-CP131单克隆抗体是原始AFS98单克隆抗体的重组嵌合型抗体。可变结构域序列与原始AFS98单克隆抗体相同，但是恒定区序列已经从大鼠IgG2a变到小鼠IgG2a。AFS98-CP131单克隆抗体像原始大鼠IgG2a抗体一样，不包含Fc突变。
AFS98-CP131单克隆抗体能与小鼠集落刺激因子1受体(CSF1R)，也称为巨噬细胞集落刺激因子受体(M-CSFR)，即CD115反应。CSF1R是一种单程I型膜蛋白，是血小板衍生生长因子受体家族的成员。在小鼠中，CSF1R由单核细胞/巨噬细胞、腹膜渗出物细胞、浆细胞样和常规树突细胞以及破骨细胞表达。CSF1R是CSF1的受体，CSF1通过CSF1R调节单核细胞谱系中细胞的增殖和分化。据报道，AFS98单克隆抗体可在体内deplete巨噬细胞并阻断CSFR1。
产品详情：
产品名称 | RecombiMAb anti-mouse CSF1R (CD115) |
产品货号 | CP131 |
产品规格 | 1/5/25/50/100mg |
反应种属 | Mouse |
克隆号 | AFS98-CP131 |
同种型 | Mouse IgG2a(switched from rat IgG2a) |
免疫原 | Not available or unknown |
实验应用 | in vivo macrophage depletion*
in vitro CSF1R neutralization*
in vivo monocyte depletion*
Flow cytometry*
Western blot*
*Reported for the original rat IgG2a AFS98 antibody |
产品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
纯度 | >95%, Determined by SDS-PAGE |
聚合 | <5%, Determined by SEC |
无菌处理 | 0.2 µm filtration |
纯化方式 | Protein A |
分子量 | 150 kDa |
小鼠病原检测 | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
保存条件 | 抗体原液保存在4°C，不能冷冻保存。 |
推荐同型对照 | InVivoPlus mouse IgG2a isotype control, unknown specificity（货号BP0085） |
推荐抗体稀释液 | InVivoPure pH 7.0 Dilution Buffer（货号IP0070） |
该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：
| 应用 | 文章 |
体内巨噬细胞耗竭 (in vivo macrophage depletion) | 1. Bauche, D., et al. (2018). 'LAG3(+) Regulatory T Cells Restrain Interleukin-23-Producing CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis' Immunity 49(2): 342-352 e345. 2. Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499. 3. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses' Nat Med. doi : 10.1038/nm.4200. 4. Naik, S., et al. (2015). 'Commensal-dendritic-cell interaction specifies a unique protective skin immune signature' Nature 520(7545): 104-108. |
体外CSF-R1中和 (in vitro CSF-R1 neutralization) | 1.Sheng, K. C., et al. (2014). 'IL-3 and CSF-1 interact to promote generation of CD11c+IL-10-producing macrophages' PLoS One 9(4): e95208. |
流式细胞术 (Flow Cytometry) | 1. Li, W., et al. (2012). 'Intravital 2-photon imaging of leukocyte trafficking in beating heart' J Clin Invest 122(7): 2499-2508. 2. Tagliani, E., et al. (2011). 'Coordinate regulation of tissue macrophage and dendritic cell population dynamics by CSF-1' J Exp Med 208(9): 1901-1916. |
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