EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的CUT&RUN级别的BRD4 Antibody符合EpiCypher的“CUTANA Compatible”标准，可用于核酸酶靶向切割和释放（CUT&RUN）和/或靶向剪切及转座酶技术（CUT&Tag）的基因组定位。BRD4抗体主要在转录起始位点的侧翼产生CUT&RUN峰(Fig. 1)。正如文献所报道，BRD4峰与BRG1/SMARCA4峰有很大程度的重叠(Fig. 2)。

产品详情

反应种属: Human, Mouse

宿主来源: Rabbit

实验应用: CUT&RUN, IHC, IP, WB

免疫原: Between amino acids 1312 and 1362

克隆性: Polyclonal

保存温度: Stable for 1 year at 4°C from date of receipt

运输温度: DO NOT FREEZE!! Frozen cold packs.

产品形式: Antigen affinity-purified antibody in Tris-citrate/phosphate buffer pH 7-8, 0.09% sodium azide

验证数据

Figure 1: BRD4 peaks in CUT&RUN

CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show BRD4 peaks relative to IgG negative control antibody and H3K4me3 positive control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. All rows were aligned relative to H3K4me3 antibody.

Figure 2: BRD4 CUT&RUN representative browser tracks

CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci show overlap of BRD4 and BRG1 peaks.

Figure 3: Immunohistochemistry data

FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) using BRD4 antibody at a dilution of 1:5,000.

Figure 4: Immunoprecipitation data

EpiCypher BRD4 antibody (6 µg) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HeLa cells. A negative control IgG antibody and positive control antibody targeting BRD4 (Bethyl Laboratories) were also used to demonstrate the specificity of the IP. For blotting immunoprecipitates, Bethyl Laboratories BRD4 antibody (A700-004) was used at a dilution of 1:1,000.

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