Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit提供了一种荧光分析方法，用于检测广泛生物靶标的细胞内钙动员情况。相对于其他商业化的染料，GFP-CERTIFIED® FLUOFORTE®染料是最亮和最灵敏的荧光钙指示剂。该试剂盒为贴壁和非贴壁细胞系提供了更少步骤，更低变异性，更简易的钙检测。此外，它既不需要洗涤步骤，也不需要添加外源性淬灭染料，避免了对受体-配体相互作用动力学的干扰。
Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit可用于细胞内钙离子的高通量检测，钙离子是重要的第二信使，常用于初步筛选中监测G蛋白偶联受体激活。
产品特色
● 可监测表达GFP细胞中的钙离子动员情况
● 最明亮、最灵敏的荧光Ca2+指示剂
● 获得的 EC50和IC50值与使用钙离子荧光探针 Rhod-4 AM和Fluo-4 AM 相当
● 更大范围的检测窗口
● 使用方便，无需洗涤，无需淬灭染料
实验示例
图2. U-2 OS细胞分别与（A）GFP-CERTIFIED® FLUOFORTE®AM染料和（B）Rhod-2 AM染料孵育。洗涤细胞并用终浓度为100nM的ATP处理，并立即成像。GFP-CERTIFIED® FLUOFORTE® AM染料比Rhod-2 AM表现出更亮的荧光强度。
产品信息
产品货号 | ENZ-51020-KP010/ ENZ-51020-KP100 |
产品名称 | GFP-CERTIFIED® FLUOFORTE® Calcium assay kit |
规 格 | 10*96wells/100*96wells |
短期保存 | -20°C |
长期保存 | -80°C |
试剂盒组分 | Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates
High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates |
部分产品引用文献
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10. Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)
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