Enzo Life Sciences的ROS-ID® NO Detection kit以红色荧光的NO检测试剂为主要成分，可通过荧光显微镜直接实时监测活细胞中NO的产生。这种非荧光的、可渗透细胞的NO检测染料在O2存在的情况下能够与NO发生反应，具有高特异性、灵敏性和准确性，产生不溶于水的红色荧光产物。这种染料对过氧亚硝酸盐没有反应性，因此可以区分过氧亚硝酸盐和一氧化氮。
ROS-ID® NO Detection kit中的荧光探针对活性氯或溴不敏感，因此不用于检测这些分析物。染色后，该染料产生的荧光产物可通过荧光显微镜进行观察。当需要同时检测额外的荧光信号（绿色或橙色）时，建议使用650/670 nm的组合。ROS-ID® NO Detection kit包含足够的试剂，可对活细胞（贴壁或悬浮）进行至少200次检测。
产品特色
通过荧光显微镜直接实时监测活体细胞中NO的产生
高特异性、灵敏性和准确性，产生不溶于水的红色荧光产物
可区分过氧亚硝酸盐和一氧化氮
试剂盒内包含一氧化氮诱导剂和清除剂
产品信息
产品货号 | ENZ-51013-200 |
产品名称 | ROS-ID® NO Detection kit |
规格 | 1*1Kit |
操作 | Protect from light. Avoid freeze/thaw cycles. |
短期保存 | -20°C |
长期保存 | -80°C |
试剂盒组分 | NO Detection Reagent (Red), 60 µl NO Inducer (L-Arginine), 100 µl NO Scavenger (c-PTIO), 400 nmoles 10X Wash Buffer, 15 ml |
应用 | Fluorescence microscopy, Fluorescent detection |
部分产品引用文献
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