CD28 (human):Fc (human) (rec.)-Fc融合蛋白-ELISA_抗体_蛋白_核苷酸

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CD28 (human):Fc (human) (rec.)

品牌：Adipogen

官网链接

说明书

折扣/货期

货号：CHI-HF-210CD28-C200

规格：200 ug

目录价：￥5740

产品详情

产品名称:

CD28 (human):Fc (human) (rec.)

产品描述:

CD28 and CTLA-4 together with their ligands, CD80 (B7-1) and CD86 (B7-2), constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is downregulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approx.y 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

宿主来源:

CHO cells

保存温度:

-20°C

运输温度:

BLUE ICE

产品形式:

solid

存储溶液:

Lyophilized from 0.2µm-filtered solution in PBS.

有效期:

Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

产地:

瑞士

纯度:

>98% (SDS-PAGE)

生物活性:

Measured by its binding ability in a functional ELISA. Immobilized CD28 (human):Fc (human) (rec.) (#CHI-HF-210CD28) at 20µg/ml (50µl/well) can bind the protein CD80 (human):Fc (mouse) (rec.) (#CHI-HF-211CD80). The EC50 is about 0.05µg/ml.

内毒素水平:

<0.06EU/µg protein (LAL test; Lonza).

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