Trident Universal Protein Blocking Reagent (animal serum free)-封闭试剂-ELISA_抗体_蛋白_核苷酸

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Trident Universal Protein Blocking Reagent (animal serum free)

品牌：GeneTex

官网链接

说明书

折扣/货期

货号：GTX30963

规格：100ml

目录价：￥1200

产品详情

产品名称:

Trident Universal Protein Blocking Reagent (animal serum free)

别名:

Blocking Solution , Blocking Buffer

产品描述:

Protein Blocking Reagent (animal serum free)

实验应用:

ELISA, ICC/IF, IHC, IHC-Fr, WB

克隆号:

产品浓度:

保存温度:

Store at 2-8ºC.

运输温度:

4°C

产品形式:

Liquid

存储溶液:

20mM Tris, 150mM NaCl, 0.02% Triton X-100, 0.05% ProClin 300.

产地:

美国

功能与背景:

Immunohistochemistry (IHC)/ Immunocytochemistry (ICC) is the localization of antigens by the use of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used: labeled biotin secondary antibody streptavidin-peroxidase (LBSASP), HRP anti-HRP, ABC, catalyzed signal amplification, polymer system and others, to detect antigens on tissue and cell In this kit the first layer is unlabeled primary antibody, the second layer is biotinylated secondary antibody, the third layer is Enzyme-Streptavidin conjugate (HRP-Streptavidin) to replace the complex of avidin-biotin peroxidase. The enzyme is then visualized by application of the substrate chromogen solution to produce different colorimetric end products.

This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays.

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