ENZO热销产品——ER-ID® Green assay kit内质网检测试剂盒（绿色）
Enzo Life Sciences公司的ER-ID® Green assay kit适用于活细胞或破膜醛固定细胞的染色。微摩尔级浓度的ER-ID®绿色染料足以对哺乳动物细胞进行染色，在人宫颈癌细胞系、T淋巴细胞系、Jurkat 细胞系、HeLa 细胞系以及骨肉瘤上皮细胞U2-OS中已经得到了验证。ER-ID® Green assay kit专门用于表达RFP或OFP的细胞系，以及表达蓝色或青色荧光蛋白（BFP、CFP）的细胞。该试剂盒适用于活细胞或固定后细胞与探针（如标记抗体）或与Texas Red或香豆素类似光谱性质的其他荧光缀合物结合使用。
产品特点
● 新型内质网染料，可用于活细胞、破膜细胞或固定细胞的染色
● 与常见荧光染料和荧光蛋白兼容
● 高耐光漂白性、抗浓度淬灭和光转换
● 生产严格，以控制和消除非特异性结果
实例分析
● ER-ID®绿色染色与含有KDEL序列的钙网蛋白共定位，与橙色荧光蛋白（OFP）融合。
● 用ER-ID®绿色染料（A）、Hoechst 33342染料（B）对HeLa活细胞进行染色，得到的合成图像（C）。
● 用4%多聚甲醛固定HepG2细胞20分钟，并用ER-ID®标记30分钟。下图为标记3天后拍摄的照片。
● 将HepG2细胞与ER-ID®孵育30分钟，并用倒置显微镜（Zeiss）进行分析。
产品详情：
产品名称 | ER-ID® Green assay kit |
产品货号 | ENZ-51025-K500 |
产品规格 | 500 assays |
质量控制 | A sample from each lot of ER-ID® Green assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivity of the ER-ID® Green dye is evident. |
应用 | Fluorescence microscopy Enzo Life Sciences’ ER-ID® Green assay kit contains a novel endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. |
长期储存 | -20°C |
组分 | ER-ID® Green detection reagent: 50μl Hoechst 33342 nuclear stain: 50μl 10X assay buffer: 15ml |
产品说明 | The ER-ID® Green assay kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents |
部分产品文献引用
1. Molecular interplay between NOX1 and autophagy in cadmium-induced prostate carcinogenesis: A. Tyagi, et al.; Free Radic. Biol. Med. 199, 44 (2023)
2. Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum: A. Komatsu, et al.; J. Biol. Chem. 298, 101933 (2022)
3. PLP1 mutations in patients with multiple sclerosis: identification of a new mutation and potential pathogenicity of the mutations: N.C. Cloake, et al.; J. Clin. Med. 7, 342 (2018), Application(s): Fluorescence microscopy
4. Englerin A induces an acute inflammatory response and reveals lipid metabolism and ER stress as targetable vulnerabilities in renal cell carcinoma: A. Batova, et al.; PLoS One 12, e0172632 (2017), Application(s): Fluorescence microscopy
5. Cell growth on (“Janus”) density gradients of bifunctional zeolite L crystals: N.S. Kehr, et al.; ACS Appl. Mater. Interfaces 8, 35081 (2016)
6. The parathyroid hormone second receptor PTH2R and its ligand tuberoinfundibular peptide of 39 residues TIP39 regulate intracellular calcium and influence keratinocyte differentiation: E. Sato, et al.; J. Invest. Dermatol. 136, 1449 (2016)
7. Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies: V. Ramanan, et al.; Virology 494, 236 (2016), Application(s): Endoplasmic reticulum staining
8. Localization-Dependent Cell-Killing Effects of Protoporphyrin (PPIX)-Lipid Micelles and Liposomes in Photodynamic Therapy: S. Tachikawa, et al.; Bioorg. Med. Chem. 23, 7578 (2015), Application(s): Cell staining
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