人血红素加氧酶1 HO-1 (human), ELISA kit—Enzo Life Sciences热销产品
血红素加氧酶-1 (HO-1)也称为Hsp32，是血红素加氧酶的诱导型异构体，对血红素的NADPH、氧和细胞色素P450还原酶依赖性氧化进行催化，氧化为一氧化碳、亚铁和胆绿素，胆绿素迅速还原为胆红素。这些反应产物具有重要的生理作用：一氧化碳是一种有效的血管扩张剂，并被认为是cGMP和血管紧张度的生理调节剂；胆绿素及其产物胆红素是有效的抗氧化剂；“游离”铁通过影响铁调节蛋白(IRP)-1的构象及其与mRNAs的5’-或3’-UTR中的铁调节元件(IREs)的结合，增加氧化应激并调节许多mRNAs(例如，DCT-1、铁蛋白和转移受体)的表达。三种已鉴定的血红素加氧酶亚型是催化血红素转化为胆绿素和一氧化碳的血红素加氧酶系统的一部分，分别是可诱导的HO-1或Hsp32，在脑和睾丸中大量存在的组成型HO-2，以及与HO-2相关但为不同基因产物的HO-3。
Enzo Life Sciences的HO-1 (human), ELISA kit提供了一种检测和定量人源样品中血红素氧合酶(HO-1)的方法。该试剂盒可以精确地测定细胞裂解物（cell lysates）和组织提取物（tissue extracts）中的HO-1，不与人HO-2或HO-3(其他已知的血红素加氧酶同种型)交叉反应。
产品特点
● 高灵敏度，可检测低至0.78 ng/ml的HO-1
● 重复可靠的检测结果
● 高通量，可在2.5h小时内完成多达40个样本的检测（含复孔）
● 优于半定量Western blot分析的全定量检测
产品信息
| 产品货号 | ADI-EKS-800 |
产品名称 | HO-1 (human), ELISA kit（人血红素加氧酶-1 ELISA试剂盒） |
规格 | 96 wells |
别名 | Heme oxygenase 1 |
灵敏度 | 0.78 ng/ml |
检测范围 | 0.78 - 25 ng/ml |
检测时间 | <2.5 hours |
适用种属 | Human |
交叉反应 | Human HO-1 (100%). No reactivity with HO-2 or HO-3. |
适用样本类型 | For the quantitative determination of human heme oxygenase-1 in cell lysates and tissue extracts. Cited sample types include serum and plasma. |
试剂盒组分 | Microtiter plate, 5X Extraction reagent, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, HRP Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2 |
标准曲线示例
部分产品文献引用
1. Does a negative correlation of heme oxygenase-1 with hematoma thickness in chronic subdural hematomas affect neovascularization and microvascular leakage? A retrospective study with preliminary validation: H.T. Luh, et al.; J. Neurosurg. (2023)
2. Delivery of self-replicating messenger RNA into the brain for the treatment of ischemic stroke: M. Kim, et al.; J. Control Release 350, 471 (2022)
3. Effects of Tolvaptan on Oxidative Stress in ADPKD: A Molecular Biological Approach: M. Rigato, et al.; J. Clin. Med. 11, 402 (2022)
4. Associations between blood heavy metal(loid)s and serum heme oxygenase-1 in pregnant women: Do their distribution patterns matter?: K. Li, et al.; Environ. Pollut. 286, 117249 (2021)
5. Dual-Functional Dendrimer Micelles with Glycyrrhizic Acid for Anti-Inflammatory Therapy of Acute Lung Injury: M. Choi, et al.; ACS Appl. Mater. Interfaces 13, 47313 (2021)
6. Induction of HO-1 by 5, 8-Dihydroxy-4,7-Dimethoxyflavone via Activation of ROS/p38 MAPK/Nrf2 Attenuates Thrombin-Induced Connective Tissue Growth Factor Expression in Human Cardiac Fibroblasts: C. Yang, et al.; Oxid. Med. Cell. Longev. 2020, 1080168 (2020)
7. Beneficial use of serum ferritin and heme oxygenase-1 as biomarkers in adult-onset Still's disease: A multicenter retrospective study: Y. Kirino, et al.; Mod. Rheumatol. 28, 858 (2018), Application(s): ELISA using human serum
8. Camel Milk Ameliorates 5-Fluorouracil-Induced Renal Injury in Rats: Targeting MAPKs, NF-κB and PI3K/Akt/eNOS Pathways: H.H. Arab, et al.; Cell. Physiol. Biochem. 46, 1628 (2018), Application(s): ELISA with Rat
9. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome: Y. Hara, et al.; Can. Respir. J. 2018, 9627420 (2018), Application(s): Human serum
10. Endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol: in vitro and in vivo studies: T. Barta, et al.; Naunyn Schmiedebergs Arch. Pharmacol. 391, 371 (2018)
11. Haemolysis and haem oxygenase-1 induction during persistent 'asymptomatic' malaria infection in Burkinabé children: J.P. Mooney, et al.; Malar. J. 17, 253 (2018)
12. Hepatoprotective Potential of Standardized Ficus Species in Intrahepatic Cholestasis Rat Model: Involvement of Nuclear Factor-κB, and Farnesoid X Receptor Signaling Pathways: S.S. El-Hawary, et al.; J. Ethnopharmacol. 231, 262 (2018)
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